The Huanggangliang Fe-Sn deposit is located in a late Paleozoic accretionary orogenic belt within the southern segment of the Da Hinggan Mountains. Two types of sphalerite occur in skarn, i.e., disseminated sphalerite and lamellar sphalerite. High-precision trace elements analysis was performed for the two types of sphalerite by LA-ICP-MS, and the results indicate that they are syngenetic. The sphalerite presents high concentrations of Mn, Cu, As and In, low concentrations of Ga, Ge and Cd, and wide-ranged concentrations of As, Sn, Bi and Pb. Cu, Sn, Bi and Pb occur as independent minerals, and Mn, Fe, Ga, Ge, Cd, In and Sb enter the lattice of sphalerite in the form of isomorphism. Low In/Ga and In/Ge ratios and Zn/Cd ratios from 233 to 250 suggest that the sphalerite was formed under a moderate-high temperature condition. Cd/Fe ratios below 0.1 and Cd/Mn ratios below 0.5 indicate that the formation of sphalerite was related to magmatism, and the In-Ge discrimination diagram of ore deposit genesis also suggests skarn type. According to comparing these results with other studies of sphalerite from typical ore deposits and in consideration of geological features of the deposit, the authors hold that the Huanggangliang Fe-Sn deposit is a moderate-high temperature skarn-type deposit related to the Yanshanian magmatism.