The Xiwanggou mafic-ultramafic complex is located in the eastern segment of the Central Kunlun Zone and the magma differentiation is sufficient with lithologies consisting of gabbro facies, pyroxenite facies, and peridotite facies. Amphibole preserves crucial information regarding magmatic evolution and the geological setting of its formation, and it is ubiquitously present across diverse lithologies within Xiwanggou complex. In this study, we use the characteristics of major and trace elements contents in amphiboles, in conjunction with whole-rock geochemistry and the major element characteristics of plagioclases, to discuss the petrogenesis and magmatic evolution of the complex. The findings offer novel constraints on the tectonic evolution of the East Kunlun spanning from the Late Paleozoic to the Early Mesozoic. Experimental results demonstrate that all the amphiboles are mainly classified as pargasite and Mg-hastingsite of magmatic origin, bearing the typical hallmarks of subduction amphiboles. Calculations indicate that the crystallization at the range of 889~971℃, 191~280 MPa, △NNO+0.2~+1.8, and the magma water content is 2.8%~4.6%. From these results, it can be inferred that during the magma evolution, it underwent the process of cooling, decompression, reduction, and water enrichment. The compositions of amphibole melt at equilibria display highly comparable trace element and rare earth element signatures. Major element contents reveal that the melt compositions associated with amphibole crystallization in pyroxene peridotite, olivine pyroxenite, and gabbro are relatively primitive, while the melts during amphibole crystallization in amphibole gabbro exhibit relatively evolved characteristics. Comprehensive analysis indicates that the diverse rocks within the Xiwanggou complex were formed during different evolution stages of homologous magma. As the magma ascended and emplaced, it progressively evolved, becoming enriched in Si and depleted in Mg, and underwent the fractional crystallization of plagioclase. These rocks thus serve as critical magmatic records, documenting the subduction of the Paleo-Tethys Ocean beneath the East Kunlun Orogen during the Early-Middle Triassic period.