The Chang'e-5 (CE5) and Chang'e-6 (CE6) missions returned lunar regolith samples from the Procellarum KREEP Terrane (PKT) on the Moon's nearside and the South Pole-Aitken Basin (SPA) on the farside, respectively, providing invaluable materials for comparative studies of geological differences between the Moon's two hemispheres. Spinel group minerals are significant accessory phases in various lunar rocks. Owing to their complex isomorphic substitution, spinel can offer unique constraints on the petrogenesis of lunar lithologies. This study systematically investigates the petrographic and compositional characteristics of spinel grains in the Chang'e samples. In CE5 basalt samples, spinel crystals predominantly occur as inclusions in olivine grains. They evolve toward titanium-iron end member with progressive magma differentiation. Spinel in CE5 breccias and impact melt fragments generally exhibit similar features to those in basalts. In CE6 basalts, spinel grains are mainly hosted by pyroxene or occur as interstitial phase coexisting with fayalite and ilmenite, rarely found in olivine. They share a similar compositional evolution trend to CE5 basalts. Moreover, CE6 samples also contain diverse types of spinels derived from distinct sources, including Mg-suites, aggregates from lunar mantle, and impact-generated fragments with nonlunar signatures. Based on spinel compositions in basalts, thermodynamic models were conducted to constrain the parental magma compositions of CE5 and CE6 low-Ti basalts. Our results indicate that compositions of bulk basalts or melt inclusions are not appropriate proxies for the real parental magma. In addition, both the model results and the trace-element analyses of spinel samples suggest the crystallization sequences of the CE5 and CE6 basalts are different. Therefore, when reconstructing magma compositions from equilibrium melt with different lunar minerals, it is essential to consider the specific stage of magmatic evolution represented by the mineral.