The Shizhushan wollastonite deposit in Jiangxi Province is the world's largest reserve of wollastonite deposits and is located in the Pingle Depression of the eastern Qinhang metallogenic belt. In order to clarify the nature of ore-forming fluids and further prospecting potential in the area, this paper divides the garnet of the deposit into early (GrtⅠ) and late (GrtⅡ) generations according to the interpenetration relationship and optical characteristics between minerals. On the basis of detailed field work, this paper systematically studied the garnet in Shizhushan deposit by using electron probe analysis (EMPA) and LA-ICP-MS in-situ microanalysis technology. The results show that the garnets in Shizhushan wollastonite deposit belong to the grossularite to andradite solid solution series. The early generation garnet (GrtⅠ) has higher Fe content and developed oscillatory zoning, while the late generation garnet (GrtⅡ) has complex zoning. GrtⅠ and GrtⅡ are depleted in large ion lithophile elements (LILE) such as K, Rb, Ba, Sr, etc., while high field strength elements (HFSE) such as Nb, Ta, Zr are relatively high, and U content is proportional to ∑REE. The ∑REE of GrtⅡ is low (12.92×10^-6~21.12×10^-6), with a flat REE distribution pattern, slightly enriched in LREE (Ce, Pr, Nd, Sm), and weak Eu anomaly. The ∑REE of GrtⅠis higher (13.78×10^-6~73.52×10^-6), and it is more enriched in LREE (Ce, Pr, Nd, Sm) than GrtⅡ. The distribution pattern of rare earth elements is "right-inclined type", and the negative Eu anomaly is obvious. GrtⅠis formed in an acid system with relatively open, high water-rock ratio and high oxygen fugacity. GrtⅡ was formed in a relatively closed intermediate-acid system with low water-rock ratio and high oxygen fugacity, while GrtⅡ is formed in a relatively closed intermediate acid system with low water rock ratio and high oxygen fugacity. In addition, the composition and trace element characteristics of garnet are compared with those of contact metasomatic metal deposits of different minerals in domestic and abroad. The results show that the deposit has the potential to form Cu, Fe and Mo ore bodies.